PROTEOMICS

The WaNPRC is a leader in the development and use of proteomic approaches for nonhuman primate and AIDS research. Proteomics capabilities include global protein abundance profiling, targeted proteomics, and the analysis of post-translational modifications. Protein quantification is obtained using ITRAQ (isobaric tags for relative and absolute quantification) labeling and by label-free intensity methods.

The open-source LabKey Computational Proteomics Analysis System provides the computational infrastructure for managing and analyzing large volumes of mass spectrometry data. This system provides resources for all upstream aspects of data handling, including peptide identification and quantification. Data management and processing is handled through open-source and in-house developed tools and Agilent’s Mass Hunter Professional.